PicoRaman M3

Timegated Raman spectrometer analyzes fluorescent samples

Fluorescence is considered the enemy of Raman spectroscopy, as it can overlay the actual Raman molecule signals to such an extent that analysis becomes impossible. The clever way out is called timegated Raman. With this new technology, Raman spectroscopy can also measure fluorescent analytes. It means that Raman analyses can now also be used where conventional Raman reaches its limits, such as in cell cultures or fermentation.
The PicoRaman M3 spectrometer has several possible applications. It can be used as a classical benchtop system in the laboratory. But it also achieves impressive results when connected to a microscope or in a high-throughput setting with a plate reader.

Advantages of timegated Raman

• Unique fluorescence handling for separation of fluorescence and specific Raman molecule signal.
• Information gain through separated fluorescence signal.
• No problems with ambient light and thermal emission.
• 532 nm laser optimized for biopharma applications.
• Optional variant for microscope or plate reader available.

Typical areas of application

The spectrometer enables a variety of different applications.

• Biopharm applications
• Chemical syntheses and polymerizations
• Fluorescent dye analysis

How does timegated Raman work?

The fluorescence and the real Raman signal have different transit times. Timegate's patented technology can separate these two signals. Using a pulsed laser and ultra-fast switched detector, the fluorescence signal is actually suppressed and not simply removed by calculation afterwards.

What makes timegated Raman so unique?

Timegated Raman generates a fluorescence-suppressed spectrum of outstanding quality and thus enables considerably more robust models (SNR, LoD/LoQ) compared to conventional Raman. Many analyses are only possible with timegated Raman, e.g. in the biopharmaceutical sector. The method also enables not only effective fluorescence suppression, but also a separate analysis of the fluorescence. The latter often carries additional information, e.g. as a marker for the cell density in a culture. Neither ambient light nor thermal emission influence the quality of timegated Raman analyses. This simplifies installation and measurement considerably.

Practicability studies and chemometric support

We support you in assessing the practicability of analytical questions, e.g. with sample measurements in our laboratory or at the customer's site. For more detailed studies or complete chemometric modeling, we work together with partners who are absolute experts in this field, e.g. S-PACT from Aachen or LabCognition from Cologne.